Apelin antibody (C-Term)

Catalog No. ABIN6657507

Supplier Product No.: 600-401-r09

This Rabbit Polyclonal antibody specifically detects Apelin in WB. It exhibits reactivity toward Human.

Quick Overview for Apelin antibody (C-Term) (ABIN6657507)

Target: Apelin (APLN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apelin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Apelin Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-r09

Supplier: Rockland

Purpose: Apelin Antibody

Cross-Reactivity (Details): Anti-Apelin antibody is directed against human Apelin protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Apelin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Apelin.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 0.5 μg/mL

Comment:

Anti-Apelin is tested for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~8.6 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Apelin

Target: Apelin (APLN)

Alternative Name: Apelin

Background:

Synonyms: APJ endogenous ligand,

Background: Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The apelin gene to Xq25-q26.3 based on sequence similarity between the apelin sequence and a chromosome X PAC clone. This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. This antibody is suitable for researchers interested in neuroscience research.

Gene Name: APLN

Gene ID: 8862

NCBI Accession: NP_059109

UniProt: Q9ULZ1

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour