CYBB antibody (Internal Region)

Catalog No. ABIN6657508

Supplier Product No.: 600-401-r16

The Rabbit Polyclonal anti-CYBB antibody has been validated for WB and IHC. It is suitable to detect CYBB in samples from Human.

Quick Overview for CYBB antibody (Internal Region) (ABIN6657508)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYBB Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-r16

Supplier: Rockland

Purpose: NOX2 Antibody

Cross-Reactivity (Details): Anti-NOX2 antibody is directed against human NOX2 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: NOX2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NOX2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-NOX2 is tested in Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65.3 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: NOX2

Background:

Synonyms: CGD91-phox,Cytochrome b(558) subunit beta,

Background: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes .As a major player in innate immune responses in neutrophils,NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling). This antibody is suitable for researchers interested in cancer research.

Gene Name: CYBB

Gene ID: 1536

NCBI Accession: NP_000388

UniProt: P04839