DDT antibody (C-Term)

Catalog No. ABIN6657521

Supplier Product No.: 600-401-r05

This anti-DDT antibody is a Rabbit Polyclonal antibody detecting DDT in WB and IHC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for DDT antibody (C-Term) (ABIN6657521)

Target: DDT (D-Dopachrome Tautomerase (DDT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DDT Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-r05

Supplier: Rockland

Purpose: DDT Antibody

Cross-Reactivity (Details): Anti-DDT antibody is directed against human DDT protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: DDT affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human DDT.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Suggested Applications: IP
Anti-DDT is tested for Immunohistochemistry-P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~12.7 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-DDT antibody (ABIN6657521)

Song, Liu, Habibie, van den Bor, Smit, Gosens, Wu, Brandsma, Cool, Haisma, Poelarends, Melgert: "D-dopachrome tautomerase contributes to lung epithelial repair via atypical chemokine receptor 3-dependent Akt signaling." in: EBioMedicine, Vol. 68, pp. 103412, (2021) (PubMed).

Target Details for DDT

Target: DDT (D-Dopachrome Tautomerase (DDT))

Alternative Name: DDT

Background:

Synonyms: D-dopachrome tautomerase,Phenylpyruvate tautomerase II

Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure. The genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. This antibody is suitable for researchers interested in stem cell research.

Gene Name: DDT

Gene ID: 1652

NCBI Accession: NP_001077861

UniProt: P30046