PIK3R2 antibody (Internal Region)

Catalog No. ABIN6657522

Supplier Product No.: 600-401-r17

This Rabbit Polyclonal antibody specifically detects PIK3R2 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for PIK3R2 antibody (Internal Region) (ABIN6657522)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PIK3R2 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-r17

Supplier: Rockland

Purpose: PIK3R2 Antibody

Cross-Reactivity (Details): Anti-PIK3R2 antibody is directed against human PIK3R2 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: PIK3R2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PIK3R2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-PIK3R2 is tested for Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~81.5 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PIK3R2

Background:

Synonyms: Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta,

Background: PIK3R2(Phosphatidylinositol 3-kinase, regulatory subunit 2), also called p85-Beta, is an enzyme that in humans is encoded by the PIK3R2 gene. The PIK3R2 gene is mapped on 19p13.11. The NS1 protein from various influenza strains also bound p85-beta, but not p85-alpha. Binding of p85-beta and activation of PI3K required tyr89 of NS1, and mutant viruses expressing NS1 with a tyr89-to-phe substitution grew more slowly in cell culture than wildtype viruses. Using mouse embryonic fibroblasts, Park et al. showed that, in addition to regulating PI3K function, p85-alpha and p85-beta regulated the function of Xbp1s, a transcription factor that orchestrates the unfolded protein response (UPR) following endoplasmic reticulum (ER) stress. Overexpression of PIK3R2 in livers of ob/ob mice increased glucose tolerance and reduced blood glucose concentrations. This antibody is suitable for researchers interested in cancer research.

Gene Name: PIK3R2

Gene ID: 5296

NCBI Accession: NP_005018

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling