SKP2 antibody (N-Term)

Catalog No. ABIN6657524

Supplier Product No.: 600-401-p70

This anti-SKP2 antibody is a Rabbit Polyclonal antibody detecting SKP2 in WB and IHC. Suitable for Human.

Quick Overview for SKP2 antibody (N-Term) (ABIN6657524)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SKP2 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-p70

Supplier: Rockland

Purpose: SKP2 Antibody

Cross-Reactivity (Details): Anti-SKP2 antibody is directed against human SKP2 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: SKP2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the N-terminal of human SKP2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-SKP2 is tested for Immunohistochemistry-P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~47.8 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2

Background:

Synonyms: Cyclin-A/CDK2-associated protein p45,F-box protein Skp2,F-box/LRR-repeat protein 1,p45skp2

Background: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas. This antibody is suitable for researchers interested in cancer research.

Gene Name: SKP2

Gene ID: 6502

NCBI Accession: NP_001230049

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases