Lamin B1 antibody (C-Term)

Catalog No. ABIN6657537

Supplier Product No.: 600-401-p62

This Rabbit Polyclonal antibody specifically detects Lamin B1 in WB and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Lamin B1 antibody (C-Term) (ABIN6657537)

Target: Lamin B1 (LMNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Lamin B1 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-p62

Supplier: Rockland

Purpose: Lamin B1 Antibody

Cross-Reactivity (Details): Anti-Lamin B1 antibody is directed against human Lamin B1 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Lamin B1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Comment:

Anti-Lamin B1 is tested for Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~66.4 kDa corresponding to the appropriate cell lysate or extract. WB tested in Human, Mouse, Rat, IHC-P (heat) and IHC-F in human and rat, ICC in human, and ICC/IF in human.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Lamin B1 antibody (ABIN6657537)

Mastej, Axen, Wary, Minshall, Wary: "A requirement for Krüppel Like Factor-4 in the maintenance of endothelial cell quiescence." in: Frontiers in cell and developmental biology, Vol. 10, pp. 1003028, (2022) (PubMed).

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin B1

Background:

Synonyms: LMNB1, LMN2, LMNB, Lamin B1 antibody

Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This antibody is suitable for researchers interested in chromatin antibody research.

Gene Name: LMNB1

Gene ID: 4001

NCBI Accession: NP_001172028.11

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis