CNN2 antibody

Catalog No. ABIN6657541

Supplier Product No.: 200-301-p26

The Mouse Monoclonal anti-CNN2 antibody has been validated for WB, ELISA and IHC. It is suitable to detect CNN2 in samples from Turkey.

Quick Overview for CNN2 antibody (ABIN6657541)

Target: CNN2 (Calponin 2 (CNN2))

Reactivity: Turkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: CP-93

Product Details anti-CNN2 Antibody

Supplier Product No.: 200-301-p26

Supplier: Rockland

Purpose: Calponin Antibody

Cross-Reactivity (Details): This product reacts with human, chicken, and turkey.

Purification: Anti-Calponin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Calponin antibody was produced in mice by repeated immunizations with turkey gizzard calponin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:5000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.25-0.5 μg/mL

Other: ICC: 1 μg/mL

Comment:

Anti-Calponin has been tested in Immunohistochemistry -P, Immunocytochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~33 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CNN2

Target: CNN2 (Calponin 2 (CNN2))

Alternative Name: CNN2

Background:

Synonyms: Calponin H2, smooth muscle, Neutral calponin

Background: Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is thought to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in some way in the regulation or modulation of contraction. This antibody is suitable for researchers interested in cardiovascular diseases, Calcium Regulation in Cardiac Cells, myometrial pathways, skin cancer research, and down syntrome.

Gene Name: CNN2

Gene ID: 1265

NCBI Accession: NP_004359

UniProt: Q99439