CD45 antibody (APC)

Catalog No. ABIN6657603

Supplier Product No.: 200-526-n84

Product Details anti-CD45 Antibody (APC)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 200-526-n84

Supplier: Rockland

Purpose: CD45R (B220) Allophycocyanin Antibody

Cross-Reactivity (Details): Cross reactivity is observed with human and Feline.

Purification: Allophycocyanin conjugated CD45R(B220) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45R.

Sterility: Sterile filtered

Immunogen: Anti-CD45R (B220) Antibody (Monoclonal) was produced by repeated immunizations with Abelson murine leukemia virus-induced pre-B tumor cells.

Clone: RA3-6B2

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD45R (B220) is tested for Flow Cytometry and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45R (B220) (PTPRC Products)

Background:

Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

Background: CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity.

Gene Name: Ptprc

UniProt: P06800

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling