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CD45 antibody (APC)

PTPRC Reactivity: Mouse FACS, IHC, IP Host: Rat Monoclonal RA3-6B2 APC
Rockland
Catalog No. ABIN6657603
Supplier Product No.: 200-526-n84
  • Target See all CD45 (PTPRC) Antibodies
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Reactivity
    • 344
    • 221
    • 83
    • 33
    • 20
    • 19
    • 16
    • 14
    • 10
    • 10
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 284
    • 166
    • 126
    • 2
    • 1
    • 1
    Rat
    Clonality
    • 458
    • 115
    • 1
    Monoclonal
    Conjugate
    • 180
    • 58
    • 53
    • 43
    • 40
    • 14
    • 11
    • 11
    • 10
    • 10
    • 10
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45 antibody is conjugated to APC
    Application
    • 487
    • 184
    • 179
    • 119
    • 119
    • 56
    • 50
    • 48
    • 42
    • 27
    • 15
    • 13
    • 13
    • 13
    • 12
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Supplier Product No.
    200-526-n84
    Supplier
    Rockland
    Purpose
    CD45R (B220) Allophycocyanin Antibody
    Cross-Reactivity (Details)
    Cross reactivity is observed with human and Feline.
    Purification
    Allophycocyanin conjugated CD45R(B220) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45R.
    Sterility
    Sterile filtered
    Immunogen
    Anti-CD45R (B220) Antibody (Monoclonal) was produced by repeated immunizations with Abelson murine leukemia virus-induced pre-B tumor cells.
    Clone
    RA3-6B2
    Isotype
    IgG2a kappa
    Labeling Ratio
    1-2
    Top Product
    Discover our top product PTPRC Primary Antibody
  • Application Notes

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    Comment

    Anti-CD45R (B220) is tested for Flow Cytometry and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.09 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
    Expiry Date
    6 months
  • Target
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Alternative Name
    CD45R (B220) (PTPRC Products)
    Background

    Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

    Background: CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity.

    Gene Name: Ptprc

    UniProt
    P06800
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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