CD45 antibody (APC)
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- Target See all CD45 (PTPRC) Antibodies
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD45 antibody is conjugated to APC
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Supplier Product No.
- 200-526-n84
- Supplier
- Rockland
- Purpose
- CD45R (B220) Allophycocyanin Antibody
- Cross-Reactivity (Details)
- Cross reactivity is observed with human and Feline.
- Purification
- Allophycocyanin conjugated CD45R(B220) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45R.
- Sterility
- Sterile filtered
- Immunogen
- Anti-CD45R (B220) Antibody (Monoclonal) was produced by repeated immunizations with Abelson murine leukemia virus-induced pre-B tumor cells.
- Clone
- RA3-6B2
- Isotype
- IgG2a kappa
- Labeling Ratio
- 1-2
- Top Product
- Discover our top product PTPRC Primary Antibody
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- Application Notes
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
- Comment
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Anti-CD45R (B220) is tested for Flow Cytometry and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.09 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
- Expiry Date
- 6 months
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- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Alternative Name
- CD45R (B220) (PTPRC Products)
- Background
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Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Background: CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity.
Gene Name: Ptprc
- UniProt
- P06800
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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