Ataxin 1 antibody (AA 164-197) (Biotin)

Catalog No. ABIN6657670

Supplier Product No.: 200-306-w69

The Mouse Monoclonal anti-Ataxin 1 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect Ataxin 1 in samples from Mouse.

Quick Overview for Ataxin 1 antibody (AA 164-197) (Biotin) (ABIN6657670)

Target: Ataxin 1 (ATXN1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ataxin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: S76-8

Product Details anti-Ataxin 1 Antibody (Biotin)

Binding Specificity: AA 164-197

Supplier Product No.: 200-306-w69

Supplier: Rockland

Purpose: Ataxin 1 Antibody Biotin

Cross-Reactivity (Details): BLAST analysis suggests that it is 100 % identical to rat and 88 % identical to human.

Purification: Anti-Ataxin 1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Ataxin 1 Antibody was produced by repeated immunization of mice with a synthetic peptide containing amino acids 164-197 of mouse Ataxin 1.

Isotype: IgG2b

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Other: ICC/IF: 1:100

Comment:

Anti-Ataxin 1 Biotin Conjugated Antibody is tested for Western Blots, Immunohistochemistry and Immunoprecipitation. Expect a band approximately ~85 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Ataxin 1

Target: Ataxin 1 (ATXN1)

Alternative Name: Ataxin 1

Background:

Synonyms: Ataxin-1, ATX1, Atxn1, D6S504E, OTTHUMP00000016065, SCA1, Spinocerebellar ataxia type 1 protein

Background: Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons.

Gene Name: Ataxin 1

Gene ID: 20238

UniProt: P54254

Pathways: Synaptic Membrane