LGI1 antibody (AA 37-113) (PE)

Catalog No. ABIN6657734

Supplier Product No.: 200-308-w73

This anti-LGI1 antibody is a Mouse Monoclonal antibody detecting LGI1 in WB, IHC and ELISA. Suitable for Mouse.

Quick Overview for LGI1 antibody (AA 37-113) (PE) (ABIN6657734)

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGI1 antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: S283-7

Product Details anti-LGI1 Antibody (PE)

Binding Specificity: AA 37-113

Supplier Product No.: 200-308-w73

Supplier: Rockland

Purpose: Lgi1 Antibody Phycoerythrin

Cross-Reactivity (Details): BLAST analysis suggests that it is 100 % identical to rat, 98 % identical to human and ~50 % identical to LGI2, LGI3, and LGI4.

Purification: Anti-LGI1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-LGI1 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-LGI1 R-PE Conjugated Antibody is suitable for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~60kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 12 months

Target Details for LGI1

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Alternative Name: LGI1

Background:

Synonyms: ADLTE, ADPAEF, ADPEAF, Epitempin 1, EPT, ETL1, IB1099, leucine rich glioma inactivated 1, OTTHUMP00000020121, OTTHUMP00000020138

Background: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist, this LGI1 antibody will recognize only the longer form. Anti-LGI1 is ideal for research in Neuroscience and Cancer.

Gene Name: LGI1

Gene ID: 56839

UniProt: Q9JIA1