STXBP1 antibody (pSer241)

Catalog No. ABIN6657792

Supplier Product No.: 600-401-x15

This Rabbit Polyclonal antibody specifically detects STXBP1 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for STXBP1 antibody (pSer241) (ABIN6657792)

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-STXBP1 Antibody

Binding Specificity: pSer241

Supplier Product No.: 600-401-x15

Supplier: Rockland

Purpose: Munc18-1 Phospho S241 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser241 Munc18-1 Antibody is directed against Munc18-1 protein phosphorylated at S421.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser241 Munc18-1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 of mouse Munc-18-1 protein conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser241 Munc18-1 Antibody is tested for Western Blots and is specific for the ~65 kDa Munc18-1 protein phosphorylated at Ser241. Immunolabeling is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: Munc18-1

Background:

Synonyms: Syntaxin-binding protein 1, Protein unc-18 homolog 1, Unc18-1, Protein unc-18 homolog A, Unc-18A

Background: Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin. Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex. The function of Munc18-1 is thought to be regulated by phosphorylation. Munc18-1 pS241 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: STXBP1

Gene ID: 20910

NCBI Accession: NP_001107041

UniProt: O08599

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport