CD16 antibody (PE)

Catalog No. ABIN6657856

Supplier Product No.: 200-508-n80

The Rat Monoclonal anti-CD16 antibody has been validated for FACS and IP. It is suitable to detect CD16 in samples from Mouse.

Quick Overview for CD16 antibody (PE) (ABIN6657856)

Target: CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD16 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 93

Product Details anti-CD16 Antibody (PE)

Supplier Product No.: 200-508-n80

Supplier: Rockland

Purpose: CD16/32 Phycoerythrin Antibody

Cross-Reactivity (Details): Cross-reactivity with CD16/32 from other sources has not been tested.

Purification: Phycoerythrin conjugated CD16/32 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD16/32.

Sterility: Sterile filtered

Immunogen: Anti-CD16/32 Antibody (Monoclonal) was produced by repeated immunizations with sorted pre-B cells.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD16/32 is tested for FLOW and useful for Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD16

Target: CD16

Alternative Name: Fcgr3

Background:

Synonyms: Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Fcgr3

Background: CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Gene Name: Fcgr3

UniProt: P08508

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process