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CD45 antibody (APC)

PTPRC Reactivity: Mouse FACS, IHC, WB, IP, FM Host: Rat Monoclonal 30-F11 APC
Rockland
Catalog No. ABIN6657859
Supplier Product No.: 200-526-n83
  • Target See all CD45 (PTPRC) Antibodies
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Reactivity
    • 344
    • 221
    • 83
    • 33
    • 20
    • 19
    • 16
    • 14
    • 10
    • 10
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 284
    • 166
    • 126
    • 2
    • 1
    • 1
    Rat
    Clonality
    • 458
    • 115
    • 1
    Monoclonal
    Conjugate
    • 180
    • 58
    • 53
    • 43
    • 40
    • 14
    • 11
    • 11
    • 10
    • 10
    • 10
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45 antibody is conjugated to APC
    Application
    • 487
    • 183
    • 179
    • 119
    • 119
    • 56
    • 50
    • 48
    • 42
    • 27
    • 15
    • 13
    • 13
    • 13
    • 12
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
    Supplier Product No.
    200-526-n83
    Supplier
    Rockland
    Purpose
    CD45 Allophycocyanin Antibody
    Cross-Reactivity (Details)
    Cross reactivity with CD45 from other sources has not been tested.
    Purification
    Allophycocyanin conjugated CD45 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.
    Sterility
    Sterile filtered
    Immunogen
    Anti-CD45 Antibody (Monoclonal) was produced by repeated immunizations with CD45 antigen.
    Clone
    30-F11
    Isotype
    IgG2b kappa
    Labeling Ratio
    1-2
    Top Product
    Discover our top product PTPRC Primary Antibody
  • Application Notes

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    IF_Microscopy_Dilution: User Optimized

    Western_Blot_Dilution: User Optimized

    Comment

    Anti-CD45 is tested for Flow Cytometry and useful for Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
    Expiry Date
    6 months
  • Target
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Alternative Name
    CD45 (PTPRC Products)
    Background

    Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

    Background: The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.

    Gene Name: Ptprc

    UniProt
    P06800
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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