CD45 antibody (APC)
-
- Target See all CD45 (PTPRC) Antibodies
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
-
Reactivity
- Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This CD45 antibody is conjugated to APC
-
Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-526-n83
- Supplier
- Rockland
- Purpose
- CD45 Allophycocyanin Antibody
- Cross-Reactivity (Details)
- Cross reactivity with CD45 from other sources has not been tested.
- Purification
- Allophycocyanin conjugated CD45 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.
- Sterility
- Sterile filtered
- Immunogen
- Anti-CD45 Antibody (Monoclonal) was produced by repeated immunizations with CD45 antigen.
- Clone
- 30-F11
- Isotype
- IgG2b kappa
- Labeling Ratio
- 1-2
- Top Product
- Discover our top product PTPRC Primary Antibody
-
-
- Application Notes
-
Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
IF_Microscopy_Dilution: User Optimized
Western_Blot_Dilution: User Optimized
- Comment
-
Anti-CD45 is tested for Flow Cytometry and useful for Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
- Expiry Date
- 6 months
-
- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Alternative Name
- CD45 (PTPRC Products)
- Background
-
Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Background: The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.
Gene Name: Ptprc
- UniProt
- P06800
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
-