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XIRP1 antibody (pSer295)

This anti-XIRP1 antibody is a Rabbit Polyclonal antibody detecting XIRP1 in ELISA and WB. Suitable for Mouse.
Rockland
Catalog No. ABIN6657875
Supplier Product No.: 600-401-x37

Quick Overview for XIRP1 antibody (pSer295) (ABIN6657875)

Target

See all XIRP1 Antibodies
XIRP1 (Xin Actin-Binding Repeat Containing 1 (XIRP1))

Reactivity

  • 17
  • 1
Mouse

Host

  • 17
Rabbit

Clonality

  • 17
Polyclonal

Conjugate

  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This XIRP1 antibody is un-conjugated

Application

  • 9
  • 8
  • 8
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB)
  • Binding Specificity

    • 7
    • 2
    • 1
    pSer295

    Supplier Product No.

    600-401-x37

    Supplier

    Rockland

    Purpose

    Xin Actin-binding Repeat-containing Protein 1 Phospho S295 (XIRP1) Antibody

    Cross-Reactivity (Details)

    Anti-Phospho-Ser295 Xin Actin-Binding Repeat Containing Protein 1 Antibody is directed against Xin Actin-Binding Repeat Containing Protein 1 phosphorylated at S295.

    Purification

    The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

    Sterility

    Sterile filtered

    Immunogen

    Anti-Phospho-Ser295 Xin Actin-Binding Repeat Containing Protein 1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse Xin Actin-binding Repeat-containing Protein 1 conjugated to KLH.

    Isotype

    IgG
  • Application Notes

    ELISA_Dilution: 1:10,000

    Western_Blot_Dilution: 1:1000

    Comment

    Anti-XIRP1pS295 Antibody has been tested by Western Blots and is specific for the ~150 kDa and ~250 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling of the XIRP1 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

    Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

    Preservative: None

    Preservative

    Without preservative

    Storage

    -20 °C

    Storage Comment

    Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

    Expiry Date

    12 months
  • Target

    XIRP1 (Xin Actin-Binding Repeat Containing 1 (XIRP1))

    Alternative Name

    Xin Actin-binding Repeat-containing Protein 1 (XIRP1)

    Background

    Synonyms: XIRP1, Cardiomyopathy-associated protein 1

    Background: XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons. The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers. Loss of mXinα (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy. Phosphorylation of Ser295 is thought to play a key role in XIRP signaling. Xin Actin-Binding Repeat Containing Protein 1 pS295 Antibody is ideal for researchers interested in cell cycle research.

    Gene Name: XIRP1

    Gene ID

    22437

    UniProt

    O70373
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