CD8 alpha antibody (PE)

Catalog No. ABIN6657880

Supplier Product No.: 200-508-n77

The Rat Monoclonal anti-CD8 alpha antibody is suitable to detect CD8 alpha in samples from Mouse. It has been validated for FACS, IHC and IP.

Quick Overview for CD8 alpha antibody (PE) (ABIN6657880)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 53-6-7

Product Details anti-CD8 alpha Antibody (PE)

Supplier Product No.: 200-508-n77

Supplier: Rockland

Purpose: CD8a Phycoerythrin Antibody

Cross-Reactivity (Details): Cross reactivity with CD8a from other sources has not been tested.

Purification: Phycoerythrin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD8a.

Sterility: Sterile filtered

Immunogen: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with Mouse thymus or spleen .

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD8a is tested in FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Background: CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Gene Name: Cd8a

Gene ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731

Pathways: TCR Signaling