CD86 antibody (APC)

Catalog No. ABIN6657892

Supplier Product No.: 200-526-n90

The Rat Monoclonal anti-CD86 antibody has been validated for FACS, IHC and FM. It is suitable to detect CD86 in samples from Mouse.

Quick Overview for CD86 antibody (APC) (ABIN6657892)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: GL-1

Product Details anti-CD86 Antibody (APC)

Supplier Product No.: 200-526-n90

Supplier: Rockland

Purpose: CD86 Allophycocyanin Antibody

Cross-Reactivity (Details): Cross reactivity with CD86 from other sources has not been tested.

Purification: Allophycocyanin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD86.

Sterility: Sterile filtered

Immunogen: Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD86 is tested for FLOW and useful for Immunohistochemistry and Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background:

Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86, Cd86

Background: Anti-CD86 reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Gene Name: Cd86

Gene ID: 12524

NCBI Accession: NP_062261

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation