TCRb antibody (PE)

Catalog No. ABIN6657907

Supplier Product No.: 200-b08-n92

The Hamster Monoclonal anti-TCRb antibody has been validated for FACS, IHC and IP. It is suitable to detect TCRb in samples from Mouse.

Quick Overview for TCRb antibody (PE) (ABIN6657907)

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TCRb antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: H57-597

Product Details anti-TCRb Antibody (PE)

Supplier Product No.: 200-b08-n92

Supplier: Rockland

Purpose: TCRbeta Phycoerythrin Antibody

Cross-Reactivity (Details): Cross reactivity with TCRbeta from other sources has not been tested.

Purification: Phycoerythrin conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta.

Sterility: Sterile filtered

Immunogen: Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with Affinity purified TCR from mouse DO-11.10 cells.

Isotype: IgG

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-TCR beta is tested for FLOW and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for TCRb

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Alternative Name: Tcrb

Background:

Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

Background: T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Gene Name: Tcrb

Gene ID: 21577