LAMP2 antibody

Catalog No. ABIN6657933

Supplier Product No.: 200-501-g10

This Rat Monoclonal antibody specifically detects LAMP2 in WB, IP, IF and FM. It exhibits reactivity toward Mouse.

Quick Overview for LAMP2 antibody (ABIN6657933)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: GL2A7

Product Details anti-LAMP2 Antibody

Supplier Product No.: 200-501-g10

Supplier: Rockland

Purpose: Lamp2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with LAMP2 from mouse and rabbit based on 100 % homology with the immunizing sequence.

Purification: Anti-LAMP2 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: LAMP2 Antibody was produced in rat by repeated immunizations with purified preparation of mouse liver lysosomal membranes.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

IF_Microscopy_Dilution: 1:500- 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-LAMP2 Antibody is tested for use in WB, IF microscopy, and IP. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: LAMP2

Background:

Synonyms: CD107b, Igp110, Igp2, Lamp2C, LampB, MAC3, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B

Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation. Anti-LAMP2 antibody is ideal for investigators involved in cytokines, growth factors and cancer research, as well as, Chaperones and Organelle Markers research.

Gene Name: LAMP2

Gene ID: 16784

NCBI Accession: NP_001017959

UniProt: P17047

Pathways: Autophagy