MFN2 antibody (AA 370-600)

Catalog No. ABIN6657970

Supplier Product No.: 200-301-w71

The Mouse Monoclonal anti-MFN2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect MFN2 in samples from Mouse.

Quick Overview for MFN2 antibody (AA 370-600) (ABIN6657970)

Target: MFN2 (Mitofusin 2 (MFN2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: S153-5

Product Details anti-MFN2 Antibody

Binding Specificity: AA 370-600

Supplier Product No.: 200-301-w71

Supplier: Rockland

Purpose: Mitofusin 2 Antibody

Cross-Reactivity (Details): BLAST analysis suggests that it is 97 % identical to rat, 92 % identical to human and ~55 % identical to Mitofusin 1.

Purification: Anti-Mitofusin 2 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Mitofusin 2 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 370-600 of mouse Mitofusin 2.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Other: ICC/IF: 1:100

Comment:

Anti-Mitofusin 2 Antibody is tested for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~90kDa on specific lysates or tissues. Does not cross react with Mitofusin 1. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: Mitofusin 2

Background:

Synonyms: CMT2A, CMT2A2, CPRP 1, CPRP1, Fzo, HSG, hyperplasia suppressor gene, Hypertension related protein 1, KIAA0214, MARF, MFN 2, Mfn2, Mitochondrial assembly regulatory factor, Mitofusin-2, Mitofusin2, Transmembrane GTPase MFN2

Background: Mitofusin-2 (Mfn2) is a membrane protein of the mitochondria and is important in mitochondrial fusion in mammalian cells. Mfn2 activity has also been implicated in mitochondrial metabolism and the loss of Mfn2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. Mfn2 has been associated with maintaining the membrane potential of the mitochondria. Mfn2 has been shown to hinder mitochondrial antiviral signaling by inhibiting production of type 1 interferons. Mfn2 is a p53-inducible target and involved in cell proliferation, apoptosis promotion and tumor suppression. Anti-Mitofusin-2 is ideal for research in Metabolic Processes and Hemostasis.

Gene Name: Mitofusin-2

Gene ID: 170731

UniProt: Q80U63

Pathways: Skeletal Muscle Fiber Development