NrCAM antibody (AA 30-845) (HRP)

Catalog No. ABIN6658005

Supplier Product No.: 200-303-w74

This Mouse Monoclonal antibody specifically detects NrCAM in WB, ELISA and IHC. It exhibits reactivity toward Mouse.

Quick Overview for NrCAM antibody (AA 30-845) (HRP) (ABIN6658005)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NrCAM antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: S364-51

Product Details anti-NrCAM Antibody (HRP)

Binding Specificity: AA 30-845

Supplier Product No.: 200-303-w74

Supplier: Rockland

Purpose: NrCAM Antibody Peroxidase

Cross-Reactivity (Details): BLAST analysis suggests that it is 96 % identical to rat, 91 % identical to human and ~50 % identical to Neurofascin.

Purification: Anti-NrCAM Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-NrCAM Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 30-845 of mouse NrCAM.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-NrCAM HRP Conjugated Antibody is suitable for Western Blots and Immunocytochemistry. Expect a band approximately ~160kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Alternative Name: NrCAM

Background:

Synonyms: Neuronal cell adhesion molecule, Bravo, hBravo, KIAA0343, MGC138845, MGC138846, Neuronal surface protein Bravo, Ng CAM related, Ng-CAM-related, NgCAM related cell adhesion molecule, NgCAM-related cell adhesion molecule, Nr CAM, Nr-CAM

Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. Anti-NrCam is ideal for research in Cancer and Neuroscience. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.

Gene Name: NrCAM

Gene ID: 319504

UniProt: Q810U4

Pathways: Regulation of Cell Size