MECP2 antibody (pSer421)

Catalog No. ABIN6658009

Supplier Product No.: 600-401-x14

The Rabbit Polyclonal anti-MECP2 antibody has been validated for WB and ELISA. It is suitable to detect MECP2 in samples from Mouse. There is 1 publication available.

Quick Overview for MECP2 antibody (pSer421) (ABIN6658009)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MECP2 Antibody

Binding Specificity: pSer421

Supplier Product No.: 600-401-x14

Supplier: Rockland

Purpose: MeCP2 Phospho S421 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser421 MeCP2 Antibody is directed against MeCP2 protein phosphorylated at S421.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser421 MeCP2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2 protein conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser421 MeCP2 Antibody is tested for Western Blots and is specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

References for anti-MECP2 antibody (ABIN6658009)

Wang, Tian, Liu, Goldstein, Bado, Zhang, Arenkiel, Li, Yang, Du, Zhao, Rowley, Wong, Gugala, Zhang: "The Osteogenic Niche Is a Calcium Reservoir of Bone Micrometastases and Confers Unexpected Therapeutic Vulnerability." in: Cancer cell, Vol. 34, Issue 5, pp. 823-839.e7, (2019) (PubMed).

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2

Background:

Synonyms: Methyl-CpG-binding protein 2, MeCp-2 protein

Background: MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MeCP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment. MeCP2 pS421 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: MECP2

Gene ID: 17257

NCBI Accession: NP_001075448

UniProt: Q9Z2D6

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane