TNNI3 antibody (pSer43)

Catalog No. ABIN6658030

Supplier Product No.: 600-401-x34

The Rabbit Polyclonal anti-TNNI3 antibody has been validated for WB and ELISA. It is suitable to detect TNNI3 in samples from Mouse.

Quick Overview for TNNI3 antibody (pSer43) (ABIN6658030)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNNI3 Antibody

Binding Specificity: pSer43

Supplier Product No.: 600-401-x34

Supplier: Rockland

Purpose: Troponin I (Cardiac) Phospho S43 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser43 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S43.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser43 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse cardiac troponin I conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser43 Troponin (cardiac) Antibody has been tested by Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 43. Immunolabeling is greatly decreased with lambda-phosphatase treatment. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Troponin I (Cardiac)

Background:

Synonyms: Troponin I cardiac muscle, Cardiac troponin I

Background: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKC-mediated depression. Troponin I pS43 Antibody is ideal for researchers interested in cancer research.

Gene Name: TNNI3

Gene ID: 21954

NCBI Accession: NP_033432

UniProt: P48787