TNNI3 antibody

Catalog No. ABIN6658031

Supplier Product No.: 100-401-x31

This anti-TNNI3 antibody is a Rabbit Polyclonal antibody detecting TNNI3 in WB and ELISA. Suitable for Mouse.

Quick Overview for TNNI3 antibody (ABIN6658031)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNNI3 Antibody

Supplier Product No.: 100-401-x31

Supplier: Rockland

Purpose: Troponin I (cardiac) Antibody

Purification: Anti-Troponin (cardiac) Antibody is a prepared sterile filter neat serum.

Sterility: Sterile filtered

Immunogen: Anti-Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a fusion protein of complete mouse cardiac troponin 1.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Western_Blot_Dilution: 1:2000 (or higher in cardiac muscle)

Comment:

Anti-Troponin (cardiac) Antibody has been tested in Western Blots and is specific for the ~25 kDa cardiac troponin I protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Troponin I (cardiac)

Background:

Synonyms: Troponin I cardiac muscle, Cardiac troponin I

Background: Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation. In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation. Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates. Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression. Troponin I Antibody is ideal for researchers interested in cancer research.

Gene Name: TNNI3

Gene ID: 21954

NCBI Accession: NP_033432

UniProt: P48787