TNNI3 antibody (pSer150)

Catalog No. ABIN6658032

Supplier Product No.: 600-401-x33

This Rabbit Polyclonal antibody specifically detects TNNI3 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for TNNI3 antibody (pSer150) (ABIN6658032)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNNI3 Antibody

Binding Specificity: pSer150

Supplier Product No.: 600-401-x33

Supplier: Rockland

Purpose: Troponin I (cardiac) Phospho S150 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser150 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S150.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser150 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 pf cardiac troponin I conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser150 Troponin (cardiac) Antibody has been tested in Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 150. Immunolabeling is greatly decreased with lambda-phosphatase treatment. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Troponin I (cardiac)

Background:

Synonyms: Troponin I cardiac muscle, Cardiac troponin I

Background: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 and is phosphorylated by AMPK at Ser23 and Ser150. Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI. Troponin I pS150 Antibody is ideal for researchers interested in cancer research.

Gene Name: TNNI3

Gene ID: 21954

NCBI Accession: NP_033432

UniProt: P48787