NEFL antibody

Catalog No. ABIN6658045

Supplier Product No.: 900-301-d83

The Mouse Monoclonal anti-NEFL antibody has been validated for WB, IHC and FM. It is suitable to detect NEFL in samples from Pig. There is 1 publication available.

Quick Overview for NEFL antibody (ABIN6658045)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: DA2

Product Details anti-NEFL Antibody

Supplier Product No.: 900-301-d83

Supplier: Rockland

Purpose: Neurofilament L Antibody

Cross-Reactivity (Details): Anti-NF-L antibody is directed against NF-L protein.

Purification: The antibody is protein G purified from concentrated clarified cell culture supernatant.

Immunogen: Anti-NF-L Monoclonal Antibody was produced by repeated immunizations with preparation of swine intermediate filaments.

Isotype: IgG1

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Neurofilament L antibody is tested for use in Western Blotting, ICC and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 68 kDa in size corresponding to the Neurofilament L proteins in Western Blot in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NEFL antibody (ABIN6658045)

Rodrigues-Amorim, Rivera-Baltanás, Del Carmen Vallejo-Curto, Rodriguez-Jamardo, de Las Heras, Barreiro-Villar, Blanco-Formoso, Fernández-Palleiro, Álvarez-Ariza, López, García-Caballero, Olivares, Spuch: "Plasma β-III tubulin, neurofilament light chain and glial fibrillary acidic protein are associated with neurodegeneration and progression in schizophrenia." in: Scientific reports, Vol. 10, Issue 1, pp. 14271, (2021) (PubMed).

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament L

Background:

Synonyms: mouse anti-Neurofilament L Antibody, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Background: NF-L antibody detects Neurofilament L. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease. Anti-Neurofilament L antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: NEFL

Gene ID: 4747