SHANK3 antibody (Internal Region)

Catalog No. ABIN6658092

Supplier Product No.: 200-301-g45

This Mouse Monoclonal antibody specifically detects SHANK3 in WB, IHC, IP, IF and FM. It exhibits reactivity toward Rat.

Quick Overview for SHANK3 antibody (Internal Region) (ABIN6658092)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: S69

Product Details anti-SHANK3 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 200-301-g45

Supplier: Rockland

Purpose: SHANK3 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with SHANK3 from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-SHANK3 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: SHANK3 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of rat Shank3.

Isotype: IgG2b

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Comment:

Anti-SHANK3 Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~190kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: SHANK3

Background:

Synonyms: Shank3, Prosap2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation. Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders.

Gene Name: Shank3

Gene ID: 59312

NCBI Accession: NP_067708

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity