Kv2.2 antibody (N-Term)

Catalog No. ABIN6658108

Product Details anti-Kv2.2 Antibody

Target: Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))

Binding Specificity: N-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Kv2.2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Kv2.2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Kv2.2 from Mouse, human, and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Kv2.2 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: Kv2.2 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to the n-terminus region of rat kv2.2.

Immunogen Type: Recombinant Protein

Clone: S37-89

Isotype: IgG2a

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-Kv2.2 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~125 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Kv2.2

Target: Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))

Alternative Name: Kv2.2 (KCNB2 Products)

Synonyms: KCNB2 antibody, si:ch211-193n21.1 antibody, Kv2.2 antibody, 9630047L19Rik antibody, BB130875 antibody, XShab12 antibody, kcnb2 antibody, KV2.2 antibody, potassium voltage-gated channel subfamily B member 2 antibody, potassium voltage-gated channel, Shab-related subfamily, member 2 antibody, potassium channel, voltage gated Shab related subfamily B, member 2 antibody, potassium voltage gated channel, Shab-related subfamily, member 2 antibody, potassium channel, voltage gated Shab related subfamily B, member 2 S homeolog antibody, KCNB2 antibody, kcnb2 antibody, LOC100051372 antibody, Kcnb2 antibody, kcnb2.S antibody

Background:

Synonyms: KCNB2, potassium voltage gated channel subfamily B member 2, CDRK, Voltage-gated potassium channel subunit Kv2.2

Background: Voltage gated channels are tetrameters composed of four alpha-subunits arranged around a central pore. Each alpha- subunit consists of six transmembrane segments with cytoplasmic NH2 and COOH-termini. Members of the KV1-KV4 subfamilies generate functional K+ channels in a homotetrameric configuration. The KV2 subfamily consists of KV2.1 and KV2.2, and both have very similar properties. Members of the KV2 subfamily are widely expressed in neuronal tissues. They have also been reported in neurons in the dorsal root ganglia.

Gene Name: Kcnb2

Gene ID: 117105

NCBI Accession: NP_446452

UniProt: G3V9F1