Kv2.2 antibody (N-Term)

Catalog No. ABIN6658108

Supplier Product No.: 200-301-g00

The Mouse Monoclonal anti-Kv2.2 antibody is suitable to detect Kv2.2 in samples from Rat. It has been validated for WB, IHC, IP and FM.

Quick Overview for Kv2.2 antibody (N-Term) (ABIN6658108)

Target: Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Kv2.2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: S37-89

Product Details anti-Kv2.2 Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-301-g00

Supplier: Rockland

Purpose: Kv2.2 Antibody

Purification: Anti-Kv2.2 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Kv2.2 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to the n-terminus region of rat kv2.2.

Isotype: IgG2a

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-Kv2.2 Antibody is tested for use in WB, IP, and IHC. Expect a band approximately ~125kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Kv2.2

Target: Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))

Alternative Name: Kv2.2

Background:

Synonyms: KCNB2, potassium voltage gated channel subfamily B member 2, CDRK, Voltage-gated potassium channel subunit Kv2.2

Background: Voltage gated channels are tetrameters composed of four alpha-subunits arranged around a central pore. Each alpha- subunit consists of six transmembrane segments with cytoplasmic NH2 and COOH-termini. Members of the KV1-KV4 subfamilies generate functional K+ channels in a homotetrameric configuration. The KV2 subfamily consists of KV2.1 and KV2.2, and both have very similar properties. Members of the KV2 subfamily are widely expressed in neuronal tissues. They have also been reported in neurons in the dorsal root ganglia.

Gene Name: Kcnb2

Gene ID: 117105

NCBI Accession: NP_446452

UniProt: Q63099