HCN4 antibody (C-Term)

Catalog No. ABIN6658119

Supplier Product No.: 200-301-f43

This Mouse Monoclonal antibody specifically detects HCN4 in WB, IHC, IF, FACS and FM. It exhibits reactivity toward Rat.

Quick Overview for HCN4 antibody (C-Term) (ABIN6658119)

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: N114-10

Product Details anti-HCN4 Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-301-f43

Supplier: Rockland

Purpose: HCN4 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with HCN4 from Human, Rat, and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-HCN4 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: HCN4 Antibody was produced in mice by repeated immunizations with a fusion protein corresponding to the C terminus region of rat HCN4.

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Comment:

Anti-HCN3 Antibody is tested for use in WB, IHC and IF microscopy. Expect a band approximately ~130kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HCN4

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Alternative Name: HCN4

Background:

Synonyms: hyperpolarization activated cyclic nucleotide gated potassium channel 4

Background: Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.

Gene Name: Hcn4

Gene ID: 59266

NCBI Accession: NP_067690

UniProt: Q9JKA7