SH3GL1 antibody (C-Term)

Catalog No. ABIN6658121

Supplier Product No.: 200-301-g41

This anti-SH3GL1 antibody is a Mouse Monoclonal antibody detecting SH3GL1 in WB, IHC, IP and FM. Suitable for Rat.

Quick Overview for SH3GL1 antibody (C-Term) (ABIN6658121)

Target: SH3GL1 (SH3-Domain GRB2-Like 1 (SH3GL1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SH3GL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: S51-1

Product Details anti-SH3GL1 Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-301-g41

Supplier: Rockland

Purpose: SH3GL1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with SH3GL1 from Mouse, human, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-SH3GL1 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: SH3GL1 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to the near c-terminal region of rat SH3GL1.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL (Perox), 1-10 μg/mL (IF)

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-SH3GL1 Antibody is suitable for use in WB, IHC and IP. Expect a band approximately ~43kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SH3GL1

Target: SH3GL1 (SH3-Domain GRB2-Like 1 (SH3GL1))

Alternative Name: SH3GL1

Background:

Synonyms: SH3 domain GRB2 like 1 protein, CNSA1, SH3P8, EEN, Endophilin-A2, EEN fusion partner of MLL, Endophilin-2, Extra eleven-nineteen leukemia fusion gene protein, SH3 domain protein 2B

Background: Endophilin-A2 is a protein that in humans is encoded by the SH3GL1 gene. Endophilin-A2 is an SH3 domaincontaining protein that is involved in the clathrin-mediated endocytosis and recycling of synaptic vesicles. Its SH3 domain lies at the C-terminus of the protein, and can bind proline-rich sequences on other endocytotic proteins. It also binds the C-terminus of the voltage-gated calcium channel in a calcium-dependent manner, which serves to enrich the endocytotic machinery at nerve termincals.

Gene Name: SH3GL1

Gene ID: 6455

NCBI Accession: NP_003016

UniProt: Q99961