GRIN2C antibody (N-Term)

Catalog No. ABIN6658125

Supplier Product No.: 600-401-d94

Product Details anti-GRIN2C Antibody

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Binding Specificity: N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-d94

Supplier: Rockland

Purpose: NMDA R2C Antibody

Cross-Reactivity (Details): Anti-NMDA R2C antibody is directed against NMDA R2C protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NMDA R2C Antibody was produced by repeated immunizations with a fusion proteins from the N-terminal region of the NR2C subunit.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 3 μL per 200 μg lysate

Immunohistochemistry_Dilution: 1:1000-2000

IF_Microscopy_Dilution: 1:1000-2000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA R2C Antibody is tested for use in Western Blotting and Immunoprecipitation and suitable for IHC and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 140 kDa in size corresponding to the NR2C subunit of the NMDA receptor. This antibody also labels the approximately 180 kDa NR2A and NR2B subunits of the NMDA receptor.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 50μL

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GRIN2C antibody (ABIN6658125)

Yoshikawa, Kan, Shirose, Watanabe, Matsuda, Ito, Kawaguchi: "Free d-Amino Acids in Salivary Gland in Rat." in: Biology, Vol. 11, Issue 3, (2022) (PubMed).

Target Details for GRIN2C

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Alternative Name: NMDA R2C (GRIN2C Products)

Background:

Synonyms: Glutamate [NMDA] receptor subunit epsilon-3, N-methyl D-aspartate receptor subtype 2C, NMDAR2C, NR2C

Background: NMDA R2C Antibody detects NMDA R2C protein. The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. The NMDAR is also potentiated by protein phosphorylation. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level. Anti-NMDA R2C Antibody is ideal for investigators involved in Neuroscience, Signal Transduction, and Cell Signaling Research.

Gene Name: GRIN2C

Gene ID: 24411

UniProt: Q00961

Pathways: Synaptic Membrane