CAMK2A antibody

Catalog No. ABIN6658151

Supplier Product No.: 200-301-f00

This anti-CAMK2A antibody is a Mouse Monoclonal antibody detecting CAMK2A in WB, IHC, ELISA, IP, IF and FM. Suitable for Rat.

Quick Overview for CAMK2A antibody (ABIN6658151)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 6G9

Product Details anti-CAMK2A Antibody

Supplier Product No.: 200-301-f00

Supplier: Rockland

Purpose: CaMKII Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with CaMKII from mouse, rat, and bovine based on 100 % homology with the immunizing sequence.

Purification: Anti-CaMKII Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: CaMKII Antibody was produced in mice by repeated immunizations with a partially purified rat CaMKII.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: 1:2000

IF_Microscopy_Dilution: 1:50

Western_Blot_Dilution: 0.1 μg/mL (1:10,000)

Comment:

Anti-CaMKII Antibody has been tested in IF, IP, WB, IHC and RIA/ELISA. Antibody is provided in PBS pH 7.4. Expect a band approximately ~50-60kDa protein, corresponding to the molecular mass of CaMKII α-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: Camk2a

Background:

Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha

Background: CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+ auto inhibitory effect and activates the kinase . This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.

Gene Name: Camk2a

Gene ID: 25400

NCBI Accession: NP_037052

UniProt: P11275

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction