AQP2 antibody (pSer261)

Catalog No. ABIN6658216

Supplier Product No.: 612-401-d08

The Rabbit Polyclonal anti-AQP2 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect AQP2 in samples from Rat.

Quick Overview for AQP2 antibody (pSer261) (ABIN6658216)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-AQP2 Antibody

Binding Specificity: pSer261

Supplier Product No.: 612-401-d08

Supplier: Rockland

Purpose: Aquaporin 2 phospho S261 Antibody

Cross-Reactivity (Details): Anti-Aquaporin 2 pS261 Antibody is directed against rat Aquaporin 2 protein phosphorylated at S261.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Aquaporin 2 pS261 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Aquaporin 2 pS261 antibody is tested for use in ICC, IHC, IP, and WB. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa AQP2 protein phosphorylated at Ser261 in the appropriate cell lysate or extract. This antibody also recognizes the glycosylated for of aquaporin 2 at approximately 37 kDa in size.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: Aquaporin 2

Background:

Synonyms: ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Background: Aquaporin 2 pS261 antibody recognizes aquaporin 2 (AQP2) which is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking. Anti-Aquaporin 2 pS261 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: AQP2

Gene ID: 25386

UniProt: P34080

Pathways: Response to Water Deprivation