GABRA1 antibody (N-Term)

Catalog No. ABIN6658221

Supplier Product No.: 600-401-d43

This Rabbit Polyclonal antibody specifically detects GABRA1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for GABRA1 antibody (N-Term) (ABIN6658221)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GABRA1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-d43

Supplier: Rockland

Purpose: GABA(A) Receptor alpha 1 Antibody

Cross-Reactivity (Details): Labeling is absent in α1-subunit knockout animals.

Purification: GABA(A) receptor alpha 1 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-GABA(A) Receptor alpha 1 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region of rat GABAA RA1 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-GABA(A) Receptor alpha 1 (Rabbit) Antibody is tested for use in Western Blot and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 1 subunit of the GABA(A) receptor in rat brain.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABA(A) Receptor alpha 1

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor. GABA(A) receptor alpha 1 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRA1

Gene ID: 29705

UniProt: P62813