GABRB3 antibody (Cytoplasmic Loop)

Catalog No. ABIN6658225

Supplier Product No.: 600-401-c79

Product Details anti-GABRB3 Antibody

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Assay (MA)

Supplier Product No.: 600-401-c79

Supplier: Rockland

Purpose: GABAA Receptor beta 3 Antibody

Cross-Reactivity (Details): Anti-GABA(A) Receptor beta 3 Antibody is directed against rat GABA(A) Receptor beta 3.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-GABA(A) Receptor beta 3 Antibody was produced by repeated immunizations with recombinant fusion protein from the cytoplasmic loop of the beta 3 subunit of rat GABAA.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: Other
Anti-GABA(A) Receptor beta 3 (Rabbit) antibody tested for use in Western Blotting and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to the beta 3 subunit of GABA A receptor in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GABRB3 antibody (ABIN6658225)

Yamaura, Kiyonaka, Hamachi: "Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding." in: Methods in enzymology, Vol. 589, pp. 253-280, (2017) (PubMed).

Yamaura, Kiyonaka, Numata, Inoue, Hamachi: "Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry." in: Nature chemical biology, Vol. 12, Issue 10, pp. 822-30, (2017) (PubMed).

Target Details for GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Alternative Name: GABA(A) Receptor beta 3 (GABRB3 Products)

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit beta-3, GABA(A) receptor subunit beta-3, Gabrb3

Background: Anti-GABA(A) Receptor beta 3 Antibody detects GABA(A) Receptor beta 3. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor. GABA(A) receptor beta 3 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRB3

Gene ID: 24922

NCBI Accession: NP_058761

UniProt: P63079

Pathways: Sensory Perception of Sound