GABRG2 antibody (pSer327)

Catalog No. ABIN6658228

Supplier Product No.: 612-401-d52

The Rabbit Polyclonal anti-GABRG2 antibody has been validated for WB. It is suitable to detect GABRG2 in samples from Rat.

Quick Overview for GABRG2 antibody (pSer327) (ABIN6658228)

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRG2 Antibody

Binding Specificity: pSer327

Supplier Product No.: 612-401-d52

Supplier: Rockland

Purpose: GABA(A) Receptor gamma 2 phospho S327 Antibody

Cross-Reactivity (Details): Anti-GABA(A) Receptor gamma 2 pS327 Antibody is directed against rat phosphorylated GABA(A) Receptor gamma 2.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-GABA(A) Receptor gamma 2 pS327 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of rat GABAA receptor gamma 2.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-GABA(A) Receptor gamma 2 pS327 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to GABA(A) receptor gamma 2 subunit phosphorylated at Ser327 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GABRG2

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Alternative Name: GABA(A) Receptor gamma 2

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, Gabrg2

Background: Anti-GABA(A) Receptor gamma 2 pS327 Antibody detects phosphorylated GABA(A) Receptor gamma 2. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma- subunit is required for benzodiazepine modulation. It has recently been suggested that PKC? regulates the sensitivity of GABAA alpha1-beta2-gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2. GABA(A) Receptor gamma2 pS327 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRG2

Gene ID: 29709, 8393403

UniProt: P18508