Tryptophan Hydroxylase 1 antibody (pSer58)

Catalog No. ABIN6658242

Supplier Product No.: 600-401-c97

This Rabbit Polyclonal antibody specifically detects Tryptophan Hydroxylase 1 in WB. It exhibits reactivity toward Rat.

Quick Overview for Tryptophan Hydroxylase 1 antibody (pSer58) (ABIN6658242)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Binding Specificity: pSer58

Supplier Product No.: 600-401-c97

Supplier: Rockland

Purpose: Tryptophan Hydroxylase 1 phospho S58 Antibody

Cross-Reactivity (Details): Anti-Tryptophan Hydroxylase pS58 Antibody is directed against phosphorylated rat Tryptophan Hydroxylase protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification and is specific for the phosphorylated protein.

Immunogen: Anti-Tryptophan Hydroxylase pS58 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.

Isotype: IgG

Application Details

Application Notes:

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Tryptophan Hydroxylase pS58 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase proteins phosphorylated at Ser58 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: Tryptophan Hydroxylase 1

Background:

Synonyms: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1

Background: Tryptophan Hydroxylase pS58 Antibody detects phosphorylated Tryptophan Hydroxylase protein. Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH.

Gene Name: TPH1

Gene ID: 24848

UniProt: P09810

Pathways: Feeding Behaviour