Dynamin 1 antibody (pSer778)

Catalog No. ABIN6658245

Supplier Product No.: 612-601-d33

This Sheep Polyclonal antibody specifically detects Dynamin 1 in WB and ELISA. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for Dynamin 1 antibody (pSer778) (ABIN6658245)

Target: Dynamin 1 (DNM1)

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Dynamin 1 Antibody

Binding Specificity: pSer778

Supplier Product No.: 612-601-d33

Supplier: Rockland

Purpose: Dynamin phospho S778 Antibody

Cross-Reactivity (Details): Anti-Dynamin pS778 antibody is directed against human Dynamin.

Purification: Anti-Dynamin pS778 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Dynamin pS778 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Dynamin pS778 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser778 in the appropriate cell lysate or extract. Anti-Dynamin pS778 antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Anti-Dynamin pS778 antibody does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Dynamin 1 antibody (ABIN6658245)

Liu, Kochlamazashvili, Puchkov, Müller, Schultz, Mackintosh, Vollweiter, Haucke, Soykan: "Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission." in: The EMBO journal, Vol. 41, Issue 9, pp. e109352, (2022) (PubMed).

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin

Background:

Synonyms: sheep anti-Dynamin pS778 Antibody, Dynamin-1, B-dynamin, D100

Background: Dynamin Antibody detects Dynamin which is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking. Anti-Dynamin pS778 Antibody is ideal for investigators involved in Cell Signaling, Cell Motility, Neuroscience and Cancer Research.

Gene Name: DNM1

Gene ID: 140694

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling