RPH3A antibody (pSer234)

Catalog No. ABIN6658252

Supplier Product No.: 612-401-e22

The Rabbit Polyclonal anti-RPH3A antibody has been validated for WB and IHC. It is suitable to detect RPH3A in samples from Rat.

Quick Overview for RPH3A antibody (pSer234) (ABIN6658252)

Target: RPH3A (Rabphilin 3A (RPH3A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RPH3A Antibody

Binding Specificity: pSer234

Supplier Product No.: 612-401-e22

Supplier: Rockland

Purpose: Rabphilin 3A Phospho S234 Antibody

Cross-Reactivity (Details): Rabphilin 3A pS234 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Purification: Anti-Rabphilin 3A pS234 antibody is directed against rat rabphilin 3A protein phosphorylated at serine 234.

Immunogen: Anti-Rabphilin 3A pS234 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:400

Western_Blot_Dilution: 1:1000

Comment:

Anti-Rabphilin 3A pS234 (Rabbit) antibody is tested for use in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 82 kDa in size corresponding to rabphilin 3A protein phosphorylated at Ser234 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: Rabphilin 3A

Background:

Synonyms: Rabphilin 3A Homolog, Rabphilin-3A, RP3A, Rph3A, Exophilin 1, Exophilin-1

Background: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes. The expression of rabphilin 3A is reduced in an animal model of Huntington's disease. Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner. Anti-Rabphilin 3A antibody is ideal for investigators involved in Signal Transduction, Cell Signaling, and Neuroscience research.

Gene Name: RPH3A

Gene ID: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis