SYT1 antibody (pSer309)

Catalog No. ABIN6658255

Supplier Product No.: 612-401-e39

The Rabbit Polyclonal anti-SYT1 antibody has been validated for WB and IHC. It is suitable to detect SYT1 in samples from Rat.

Quick Overview for SYT1 antibody (pSer309) (ABIN6658255)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYT1 Antibody

Binding Specificity: pSer309

Supplier Product No.: 612-401-e39

Supplier: Rockland

Purpose: Synaptotagmin phospho S309 Antibody

Cross-Reactivity (Details): Anti-Synaptotagmin 1 pS309 Antibody is directed against rat Synaptotagmin 1 phosphorylated at pS309

Purification: Synaptotagmin antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Synaptotagmin pS309 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of Synaptotagmin.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:400

Western_Blot_Dilution: 1:1000

Comment:

Anti-Synaptotagmin pS309 antibody is tested for use in Western Blotting and IHC. Expect a band of approximately 60-62 kDa in size corresponding to synaptotagmin protein phosphorylated at Ser309 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Syt1

Background:

Synonyms: SytI, p65

Background: Synaptotagmin 1 pS309 Antibody detects Synaptotagmin 1, which is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS. It is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Recent studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion. It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission. Anti-Synaptotagmin 1 pS309 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: SYT1

Gene ID: 25716

UniProt: P21707

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport