NSF antibody (C-Term)

Catalog No. ABIN6658267

Supplier Product No.: 612-401-e01

The Rabbit Polyclonal anti-NSF antibody has been validated for WB. It is suitable to detect NSF in samples from Rat.

Quick Overview for NSF antibody (C-Term) (ABIN6658267)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NSF Antibody

Binding Specificity: C-Term

Supplier Product No.: 612-401-e01

Supplier: Rockland

Purpose: NSF Antibody

Cross-Reactivity (Details): Anti-NSF antibody is directed against rat N-ethylmaleimide sensitive fusion protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NSF Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region.

Isotype: IgG

Application Details

Application Notes:

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NSF Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to the NSF protein in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background:

Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF

Background: NSF Antibody detects N-ethylmaleimide sensitive fusion protein. NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes. Anti-NSF Antibody is ideal for investigators involved in Cell Signaling and Enzyme research.

Gene Name: NSF

Gene ID: 60355

NCBI Accession: NP_068516