YWHAB antibody (C-Term)

Catalog No. ABIN6658270

Supplier Product No.: 612-401-d01

The Rabbit Polyclonal anti-YWHAB antibody has been validated for WB. It is suitable to detect YWHAB in samples from Rat.

Quick Overview for YWHAB antibody (C-Term) (ABIN6658270)

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YWHAB Antibody

Binding Specificity: C-Term

Supplier Product No.: 612-401-d01

Supplier: Rockland

Purpose: 14-3-3 Antibody

Cross-Reactivity (Details): Anti-14-3-3 Antibody is directed against rat 14-3-3 protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-14-3-3 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues from the C-terminal region.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

14-3-3 antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to 14-3-3 protein in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Alternative Name: Ywhab

Background:

Synonyms: 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1

Background: 14-3-3 Antibody detects 14-3-3 protein. 14-3-3 is member of a family of highly conserved proteins that appear to have multiple roles in cell signaling. 14-3-3 is abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. Anti-14-3-3 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: YWHAB

Gene ID: 56011

NCBI Accession: NP_062250

UniProt: P35213

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location