rRNA 2'-O-Methyltransferase Fibrillarin (FBL) antibody

Catalog No. ABIN6658299

Supplier Product No.: 900-301-d39

This Mouse Monoclonal antibody specifically detects in IHC, WB and FM. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for rRNA 2'-O-Methyltransferase Fibrillarin (FBL) antibody (ABIN6658299)

Target: rRNA 2'-O-Methyltransferase Fibrillarin (FBL)

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM)

Clone: 38F3

Product Details

Supplier Product No.: 900-301-d39

Supplier: Rockland

Purpose: Fibrillarin Antibody

Cross-Reactivity (Details): Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate.

Purification: Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein.

Immunogen: Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.

Isotype: IgG1

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Anti-Fibrillarin (Mouse) Antibody is tested for use in Western Blotting, ICC and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: rRNA 2'-O-Methyltransferase Fibrillarin (FBL)

Alternative Name: NOP1

Background:

Synonyms: mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1

Background: Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

Gene Name: NOP1

Gene ID: 851548

UniProt: P15646