YDJ1 antibody

Catalog No. ABIN6658300

Supplier Product No.: 200-301-e58

The Mouse Monoclonal anti-YDJ1 antibody is suitable to detect YDJ1 in samples from Saccharomyces cerevisiae. It has been validated for ELISA, IP and WB.

Quick Overview for YDJ1 antibody (ABIN6658300)

Target: YDJ1

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Clone: 1G10-H8

Product Details

Supplier Product No.: 200-301-e58

Supplier: Rockland

Purpose: Hsp40 (YDJ1) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp40 from yeast based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp40, YDJ1 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Hsp40 (YDJ1) Antibody was produced in mice by repeated immunizations raised against full length protein yeast Hsp40 (YDJ1).

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Comment:

Anti-Hsp40 Antibody has been tested by western blot and IP and is suitable for ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 50 % (v/v) Glycerol ,0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: YDJ1

Background:

Synonyms: Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding, type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1.

Gene Name: YDJ1

Gene ID: 855661

NCBI Accession: NP_014335

UniProt: P25491