YDJ1 antibody

Catalog No. ABIN6658301

Supplier Product No.: 200-301-f59

The Mouse Monoclonal anti-YDJ1 antibody (Clone 2A7-H6) (ABIN6658301) specifically detects YDJ1 in ELISA, IP and WB. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for YDJ1 antibody (ABIN6658301)

Target: YDJ1

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Clone: 2A7-H6

Product Details

Supplier Product No.: 200-301-f59

Supplier: Rockland

Purpose: Hsp40 (YDJ1) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp40, YDJ1 from yeast based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp40, YDJ1 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Hsp40 (YDJ1) Antibody was produced in mice by repeated immunizations raised against full length protein Hsp40 (YDJ1).

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Comment:

Anti-Hsp40, YDJ1 Antibody is tested for use in WB, IP, and ELISA. Expect a band approximately ~40kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: YDJ1

Background:

Synonyms: Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.

Gene Name: YDJ1

Gene ID: 855661

NCBI Accession: NP_014335

UniProt: P25491