TIMP1 antibody (AA 103-207)

Catalog No. ABIN668331

This anti-TIMP1 antibody is a Rabbit Polyclonal antibody detecting TIMP1 in WB, ELISA, IF (p), IHC (p), IF (cc) and IHC (fro). Suitable for Human, Rat, Mouse and Rabbit. This Primary Antibody has been cited in 9+ publications.

Quick Overview for TIMP1 antibody (AA 103-207) (ABIN668331)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TIMP1 Antibody

Binding Specificity: AA 103-207

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TIMP-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TIMP1 antibody (ABIN668331)

Liang, Xiao, Long, Liu, Li, Liu, Yang: "Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy." in: International journal of molecular medicine, Vol. 40, Issue 6, pp. 1781-1791, (2018) (PubMed).

Varghese, Joseph, S R, B S, Sreelekha: "The inhibitory effect of anti- tumor polysaccharide from Punica granatum on metastasis." in: International journal of biological macromolecules, Vol. 103, pp. 1000-1010, (2017) (PubMed).

Sassoli, Chellini, Squecco, Tani, Idrizaj, Nosi, Giannelli, Zecchi-Orlandini: "Low intensity 635 nm diode laser irradiation inhibits fibroblast-myofibroblast transition reducing TRPC1 channel expression/activity: New perspectives for tissue fibrosis treatment." in: Lasers in surgery and medicine, Vol. 48, Issue 3, pp. 318-32, (2016) (PubMed).

Lu, Huang, Shih, Chang, Tsai, Lin, Shih: "Dipeptidyl peptidase-4 inhibitor decreases abdominal aortic aneurysm formation through GLP-1-dependent monocytic activity in mice." in: PLoS ONE, Vol. 10, Issue 4, pp. e0121077, (2015) (PubMed).

Ni, Ding, Zhou, Qiu, Tang: "Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats." in: European journal of pharmacology, Vol. 764, pp. 448-456, (2015) (PubMed).

Watts, Bilska, Diab, Dunster, Bulmer, Barnett, Fraser: "Novel 24-h ovine model of brain death to study the profile of the endothelin axis during cardiopulmonary injury." in: Intensive care medicine experimental, Vol. 3, Issue 1, pp. 31, (2015) (PubMed).

Sassoli, Nosi, Tani, Chellini, Mazzanti, Quercioli, Zecchi-Orlandini, Formigli: "Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells." in: Experimental cell research, Vol. 323, Issue 2, pp. 297-313, (2014) (PubMed).

Lu, Cao, Liu, Li, Chen, Fu, Zhang, Liu, Luo, Wang, Li, Caterson: "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." in: Cells, tissues, organs, Vol. 196, Issue 3, pp. 241-50, (2012) (PubMed).

Jiang, Zhou, Liu: "Combined effects of the ATP-sensitive potassium channel opener pinacidil and simvastatin on pulmonary vascular remodeling in rats with monocrotaline-induced pulmonary arterial hypertension." in: Die Pharmazie, Vol. 67, Issue 6, pp. 547-52, (2012) (PubMed).

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP-1

Background:

Synonyms: EPA, EPO, HCI, CLGI, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1

Background: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP1, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro, but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

Gene ID: 7076

UniProt: P01033