Osteocalcin antibody (AA 21-80)

Catalog No. ABIN669036

This anti-Osteocalcin antibody is a Rabbit Polyclonal antibody detecting Osteocalcin in WB, ELISA, IHC (p), FACS, IHC (fro), IF (p) and IF (cc). Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Osteocalcin antibody (AA 21-80) (ABIN669036)

Target: Osteocalcin (BGLAP)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteocalcin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Osteocalcin Antibody

Binding Specificity: AA 21-80

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Osteocalcin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Osteocalcin antibody (ABIN669036)

Zhang, Men, Fu, Shan, Ye, Wu, Tao, Liu, Jiang: "Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones." in: Cell and tissue research, Vol. 350, Issue 3, pp. 425-37, (2013) (PubMed).

Gao, Zhao, Song, Yang: "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." in: Molecular biology reports, Vol. 39, Issue 5, pp. 5791-804, (2012) (PubMed).

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Osteocalcin

Background:

Synonyms: OC, BGP, OCN, Osteocalcin, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGLAP

Background: Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2 % of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Gene ID: 632

UniProt: P02818