CD45 antibody (AA 1210-1304)

Catalog No. ABIN669606

Product Details anti-CD45 Antibody

Key Features:

• High quality CD45 Primary Antibody for the detection of PTPRC.
• Reliable product with high standard validation data

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Binding Specificity: AA 1210-1304

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Specificity: The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD45

Isotype: IgG

Product Specific Information:

What can the CD45 Antibody ABIN669606 be used for?: This polyclonal CD45 Antibody detects CD45. The CD45 Antibody has been validated for various applications and can be used for the detection of CD45 and derivatives by Flow Cytometry, Western Blotting, Immunohistochemistry (Paraffin-embedded Sections), ELISA, Immunofluorescence (Paraffin-embedded Sections), Immunofluorescence (Cultured Cells).

What validation data is available for this CD45 Antibody?: The primary antibody is referenced in 5 publications and is characterized by a proven, very high reliability. It has currently 4 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. CD45 Antibody for the detection of CD45 and derivatives.

What is the function of CD45?: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012].

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD45 antibody (ABIN669606)

Zhu, Meng, Wang, Wang, Zhang, Li, Li, Tan, Yang, Huang: "Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3201-3207, (2018) (PubMed).

Liao, Zhong, Liu, Li, Ling, Zou: "Bone mesenchymal stem cells co-expressing VEGF and BMP-6 genes to combat avascular necrosis of the femoral head." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 954-962, (2018) (PubMed).

Tsang, Huang, Yang, Chi, Chiang, Wang, Andocs, Szasz, Li, Chi: "Improving immunological tumor microenvironment using electro-hyperthermia followed by dendritic cell immunotherapy." in: BMC cancer, Vol. 15, pp. 708, (2015) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Zeng, Hu, Yuan, Hu, Zhong, Kang: "Increased expression of importin13 in endometriosis and endometrial carcinoma." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 18, Issue 6, pp. CR361-7, (2012) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 (PTPRC Products)

Background:

Synonyms: LCA, LY5, B220, CD45, L-CA, T2, CD45R, GP18, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC

Background: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Gene ID: 5788

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling