CKM antibody (AA 3-381)

Catalog No. ABIN6719307

This anti-CKM antibody is a Rabbit Polyclonal antibody detecting CKM in WB, IHC and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for CKM antibody (AA 3-381) (ABIN6719307)

Target: CKM (Creatine Kinase, Muscle (CKM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CKM Antibody

Binding Specificity: AA 3-381

Purpose: Anti-Creatine Kinase MM/CKM Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Creatine Kinase MM/CKM Antibody Picoband® (ABIN6719307). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Creatine Kinase MM recombinant protein (Position: F3-K381).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bailly, J., MacKenzie, A. E., Leblond, S., Korneluk, R. G. Assessment of a creatine kinase isoform M defect as a cause of myotonic dystrophy and the characterization of two novel CKMM polymorphisms. Hum. Genet. 86: 457-462, 1991. 2. Dawson, D. M., Eppenberger, H. M., Eppenberger, M. E. Multiple molecular forms of creatine kinases. Ann. N.Y. Acad. Sci. 151: 616-626, 1968.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: CKM

Background:

Synonyms: Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type , CPK-M, M-CK, CKM, CKMM

Background: Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Molecular Weight: 43 kDa

UniProt: P06732