Lamin B Receptor antibody (AA 102-209)

Catalog No. ABIN6719340

This anti-Lamin B Receptor antibody is a Rabbit Polyclonal antibody detecting Lamin B Receptor in WB, IHC, ELISA, FACS, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for Lamin B Receptor antibody (AA 102-209) (ABIN6719340)

Target: Lamin B Receptor (LBR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Lamin B Receptor Antibody

Binding Specificity: AA 102-209

Purpose: Anti-Lamin B Receptor/LBR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lamin B Receptor/LBR Antibody Picoband® (ABIN6719340). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lamin B Receptor/LBR recombinant protein (Position: H102-F209).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Holmer L, Pezhman A, Worman HJ (December 1998). "The human lamin B receptor/sterol reductase multigene family". Genomics. 54 (3): 469-76. 2. Schuler E, Lin F, Worman HJ (April 1994). "Characterization of the human gene encoding LBR, an integral protein of the nuclear envelope inner membrane". The Journal of Biological Chemistry.269 (15): 11312-7.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Lamin B Receptor

Target: Lamin B Receptor (LBR)

Alternative Name: LBR

Background:

Synonyms: Lamin-B receptor, Integral nuclear envelope inner membrane protein, LMN2R, LBR

Tissue Specificity: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.

Background: Lamin-B receptor is a protein, and in humans, it is encoded by the LBR gene. It is mapped to 1q42.12. The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Molecular Weight: 65-71 kDa

Gene ID: 3930

UniProt: Q14739