RNF31 antibody (AA 970-1072)

Catalog No. ABIN6719368

This Rabbit Polyclonal antibody specifically detects RNF31 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RNF31 antibody (AA 970-1072) (ABIN6719368)

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF31 Antibody

Binding Specificity: AA 970-1072

Purpose: Anti-RNF31/HOIP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RNF31/HOIP Antibody Picoband® (ABIN6719368). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF31/HOIP recombinant protein (Position: R970-K1072).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Ikeda, F., Deribe, Y. L., Skanland, S. S., Stieglitz, B., Grabbe, C., Franz-Wachtel, M., van Wijk, S. J. L., Goswami, P., Nagy, V., Terzic, J., Tokunaga, F., Androulidaki, A., Nakagawa, T., Pasparakis, M., Iwai, K., Sundberg, J. P., Schaefer, L., Rittinger, K., Macek, B., Dikic, I. SHARPIN forms a linear ubiquitin ligase complex regulating NF-kappa-B activity and apoptosis.Nature 471: 637-641, 2011.  2. Thompson, H. G. R., Harris, J. W., Lin, L., Brody, J. P. Identification of the protein Zibra, its genomic organization, regulation, and expression in breast cancer cells. Exp. Cell Res. 295: 448-459, 2004. 

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RNF31

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Alternative Name: RNF31

Background:

Synonyms: E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, HOIP, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, Zinc in-between-RING-finger ubiquitin-associated domain protein, RNF31, ZIBRA

Tissue Specificity: Expressed in both normal and transformed breast epithelial cell lines.

Background: RING finger protein 31 is a protein that in humans is encoded by the RNF31 gene. It is mapped to 14q12. The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 120 kDa

Gene ID: 55072